Species: Homo sapiens
Position: chr8: 122692221-122692545
21A is one of a number of RNAP III-transcribed ncRNAs that have sequence complementarity to protein-coding genes, possibly acting as a natural trans-chromosomal antisense RNA. Aligned to the human genome, it shows several homology hits, among which the most highly significant were associated to multiple intronic regions of centromeric protein F gene (CENP-F). In vitro over-expression of 21A transcripts inhibits CENP-F protein accumulation and decreased levels of CENP-F mRNA, indicating that 21A regulates CENP-F expression in trans. 21A RNAs has a role in the control of the proliferation of human tumor cell lines, possibly via regulation of CENP-F expression. This effect was not observed in a mouse cell line.
|sgRNA_ID||Sequence||Position (Chr)||Position (Lnc)||Length||PAM||Type||Validity||Cell line||Note||Ref.|
|sgRNA1||TATTTCTTCCATCACCATAA||122692662-122692681(-)||up stream||20||AGG||CRISPRko||High activity||HCT116||16.3-fold increase,paried with sgRNA2|||
|sgRNA2||AAGTGTTTGAATTAGCAGGT||122692175-122692194(+)||down stream||20||GGG||CRISPRko||High activity||HCT116||16.3-fold increase,paried with sgRNA1|||