CRISPRlnc: a manually curated database of validated CRISPR/Cas9 sgRNAs for lncRNAs
The first step of CRISPR/Cas9 gene editing is to design a single guide RNAs (sgRNA) to target your gene of interest. However, because sgRNAs vary widely in their activity and models, designing a sgRNA is not easy with an unwarrantable effectiveness. Thus, it is worthy of collecting validated sgRNAs, to assist in efficiently choosing sgRNA with an expected activity.
CRISPRlnc is a manually curated database of validated CRISPR/Cas9 sgRNAs for lncRNAs from all species. After manually reviewing more than 200 published literature, CRISPRlnc collected hundreds of lncRNAs and thousands of validated sgRNAs. We handled the ID, position in the genome, sequence and functional description of these lncRNAs, as well as the sequence, PAM motif, CRISPR type and validity of these sgRNAs.
Until now, tools with various characteristics for ab initio designing sgRNA are crowded, whereas systematic collection of validated sgRNA is rare. As the first database against the validated sgRNAs of lncRNAs, CRISPRlnc will provide a new and powerful approach for genome editing of lncRNAs. We believed that our CRISPRlnc database will thrive in the fields related to lncRNA and CRISPR/Cas9 studies, and will be popular among people in these fields.
Wen Chen, Ph.D. Candidate
State Key Laboratory of Developmental Biology of Freshwater Fish, School of Life Sciences, Hunan Normal University.
Prof. Changning Liu, Ph.D.
CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences.